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The consensus often points to the necessity of keeping mineral levels in check

DOI: 10.1074/jbc.272.50.31265 11

For anterograde tracing of the PVN DMV circuit, rAAV-hSyn-Cre-mCherry-WPRE-hGH polyA (AAV2/1-hSyn-Cre-mCherry, AAV2/1, 1.03 10 13 vg/mL) virus was injected into the PVN and Cre-dependent rAAV-EF1-DIO-mCherry-WPRE-hGH pA (AAV-DIO-mCherry, AAV2/9, 5.31 10 12 vg/mL) virus was injected into the DMV of C57 mice

Compared with unmodified disks carrying plasmid DNA, nanodisks modified with L17E demonstrated enhanced endosomal escape and improved transfection efficiency in cell culture