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We also observed that 90% of the enzyme activity was lost when reactions were conducted in a DTT-containing (100 mM) reaction solution, likely because glycine oxidation cannot proceed and/or C281 is fully reduced (underscoring the essentiality of the post-translational modification) (Supplementary Table 3)

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Bhatia, MD, FAAD Diana Bolotin, MD, PhD, FAAD Ivan Camacho, MD, FAAD Gary Goldenberg, MD, FAAD Alice B

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