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FIGURE 2 Epigenetic modifications in key genes related to stress and immunity, such as nuclear receptor subfamily three group C member 1 (NR3C1), TNF, and nuclear factor kappa B (NF-B), modulate both the negative feedback of the HPA axis and inflammatory pathways mediated by NF-B, suggesting a link between endocrine dysregulation and immune activation (Palma-Gudiel et al., 2021)

As a result, oxidative stress plays a critical role in both male fertility and reproductive outcomes, influencing sperm quality and the ability to conceive

Several in-vitro and in-vivo studies confirmed important effect of PEGylation on NP biodistribution, stabilization, and structure [20, 30,31,32]

Because the ADC strands are design to preferentially hybridize with cellular miRNAs, the encapsulated siRNAs are released from core/shell nanoparticles into the nucleoplasm owing to strand displacement reactions (step V) and enter the RNAi pathway, knocking-down the expression of tumor-promoting genes

Cells were then centrifuged aerobically at 4C