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Mahmud M, et al

doi: 10.3389/fphar.2021.627533

Sequencing was performed on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles (single read)

With increasing serine levels, serine functions as an allosteric activator of PKM2, supporting aerobic glycolysis and regulating either pyruvate conversion to lactic acid or pyruvate catabolism into the tricarboxylic acid (TCA) cycle [22, 23]

In contrast to previous reports suggesting that metformin activates AMPK to inhibit IIRI-induced autophagy [24, 25], our study revealed no significant change in AMPK expression following metformin treatment