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Cytotoxicity in mammalian cells Vero cells (African Green Monkey Kidney cells, ATCC CCL-81) were grown in Dulbeccos modified Eagles medium (DMEM) containing glucose, L-glutamine and sodium pyruvate and supplemented with 10% fetal bovine serum and 1X penicillinstreptomycin (Sigma) at 37 C in culture flasks (TPT-90025) until confluent growth was attained

The carboxyl (pK a 1.7), sulfonic (pK a 1.5), sulfhydryl (pK a 8.3), and amino (pK a 9 to 11) groups all undergo ionization

To further support these findings, we also detected activated caspases 3/7, an apoptotic marker, using a fluorescent dye (CellEvent