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the only sample preparation required may be the decanting of the conditioned media from adherent cells or low-speed centrifugation to remove suspension cells

After each PTZ injection, each rat was observed for 30 min for latency to an epileptic fit, duration of seizure, and seizure stage according to a modified Racine scale (Giardina and Gasior 2009) as follows: stage 0, no response

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