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Note: Any mention of "baked asparagus" is an amateur reference to roasting

When hPSCs reached approximately 70% confluency, the culture medium was switched to RPMI1640 supplemented with 1 B27

2022 Jinqiang Zhu, Wanshan Song, Lin Li and Xiang Fan, Abstract Endothelial nitric oxide synthase: A potential therapeutic target for cerebrovascular diseases, Molecular Brain (2016) 9:30 He M, Wang D, Xu Y, Jiang F, Zheng J, Feng Y, Cao J, Zhou X

The results robustly demonstrated that the synergistic combination of curcumin with complementary agents exerts pleiotropic therapeutic effects in DED, addressing multiple pathophysiological pathways simultaneously

After staining, the brain tissue images were taken using a microscope, and the number of normal neurons and dark neurons in the cerebral cortex and hippocampus (CA1, CA3, DG) were counted using ImageJ software