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The experience of a Portuguese Pediatric Centre

In contrast, when neurospheres were co-cultured with HT-22 cells pretreated with high glucose, the differentiated immature neurons showed shortened neurites with reduced MAP2-positive areas, although the number of precursor cells remained unchanged

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Neutralization of Oxidative Stress Markers

After washing several times with PBST, proteins were detected using the Pierce ECL western blotting substrate (Thermo Scientific)