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Briefly, the plasmids were diluted in serum-free medium was mixed with PEI (1 g/l)/TurboFect at a ratio of 1:3 (DNA amount: Transfection reagent volume), followed by incubation for 1520 min at room temperature and then added to cells

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For example, silencing the lncRNA MEG3 in chondrocytes increased miR-885-5p expression while downregulating SLC7A11 and GPX4, thereby making chondrocytes more sensitive to ferroptosis (Zhu et al., 2024)