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The presence of cell surface proteins, ion channels, efflux pumps, enzymes, specific receptors, and transporters on pericytes (PCs), vascular smooth muscle cells (VSMCs), and ECs maintain BBB integrity (Sweeney et al., 2019b) and allow bidirectional regulation of substances by transcellular or paracellular transport (Haseloff et al., 2015

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After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

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Prime Penetration: Often cited at 5060% of households, though some sources differ