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At any rate, the long-term effects of hydrochlorothiazide treatment on essential metabolic parameters such as body weight, body composition and dynamic evaluations of glucose homeostasis and metabolomics have not yet been conducted

NTS were purified by layering on 4 C Percoll gradient suspension containing Eagles minimum essential medium, penicillin-streptomycin (10,000 U per ml penicillin/10,000 U per ml streptomycin), and 1 M HEPES in 0.85% NaCl with cercariae suspension and centrifuged at 500 g for 15 min

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Such combined inhibition might avoid some disadvantages of selective COX-2 inhibitors and spares the gastrointestinal mucosa