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Likewise, homocysteine is also HIGH if you are B12 deficient

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Mf were cleaned and filtered in RPMI 1640 culture media (Gibco, Thermo Fisher Scientific) using PD-10 desalting columns (GE Healthcare) supplemented with 2 mM L-glutamine, 25 mM Hepes, 100 U/mL penicillinstreptomycin (Thermo Fisher Scientific) and 2.5 mg/mL amphotericin B (Thermo Fisher Scientific) at 37 C