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THEO VON: But do you think that they all know that

As a prominent researcher, Michael Russell, wrote, People smoke for nicotine but they die from the tar

To determine the levels of mRNA expression, the first-strand cDNA was amplified using KAPA SYBR FAST Universal (KAPA Biosystems) on a BioRad CFX384 Real-Time System, BioRad, California, USA

Cannabinoids exert their effects primarily through activation of the ECS, which consists of the cannabinoid receptors CB1 and CB2, endogenous ligands such as anandamide and 2-arachidonoylglycerol (2-AG), and enzymes responsible for ligand synthesis and degradation (50)