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Generation of Alas1 -/- preadipocytes Alas1 gRNAs were cloned into the backbone of PX459 plasmid backbone as described previously 48

Drug evaluation: tesamorelin, a synthetic human growth hormone releasing factor

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In particular, some studies report the ratio of free GSH to GSSG [19, 22,23,24,25,26], whereas others quantify and report total GSH (i.e., free GSH + 2xGSSG) [12, 19, 24, 26,27,28,29,30]