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In addition to that, the density of cortical stromal cells was markedly decreased, and vacuolated areas without stromal cells were detected between the atretic follicles

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S4 MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 2.44 mM glucose (Merck Millipore, USA), 29 mM sodium bicarbonate (Merck Millipore, USA), 2% BSA (Capricorn Scientific, USA), 500 l/100 mL ITS-X (Thermo Fisher Scientific, Netherlands), 5 g/mL heparin sulfate (Sigma Aldrich, USA), S5 MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 2.44 mM glucose (Merck Millipore, USA), 2% BSA (Capricorn Scientific, USA), 260 nM zinc sulfate (Merck Millipore, USA), 5 g/mL heparin sulfate (Sigma Aldrich, USA), 1x Trace Elements A (Corning, USA), 1x Trace Elements B (Corning, USA)