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4.3.11 Brown spot Physoderma maydis is the cause of brown spot disease, is a widespread affliction that commonly appears around the tasseling stage of maize

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The cassette was assembled with overlap extension PCR and transformed into ADP1 WT resulting in strain ASA1001

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After transformation with the rescue cassette, the cell culture was diluted 1:10, 1:100 and 100 l volumes were spread on LA plates supplemented with 400 g/ml zidovudine and 50 mM glucose