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EVs isolated from 1 mL of the supernatant were resuspended in 100 L of PBS

39 Hence, other studies have reported conflicting results, 40 highlighting the complexity of glucose metabolism in ARDS

HP -glutamyl-[1- 13 C]glycine has been used to evaluate GGT activity in kidneys and in a subcutaneous ovarian carcinoma xenograft model 31,32,33

Basal insulin was measured in plasma from female [Young ( n = 16), Old Con ( n = 26), and Old mAb ( n = 25)] and male mice [Young ( n = 17), Old Con ( n = 32), and Old mAb ( n = 28)] using a bead-based assay for mouse insulin (EMD Millipore, Inc) and detection was performed on a Bio-Plex MAGPIX Multiplex Reader (Biorad Inc., Hercules, CA)