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Key challenges include high manufacturing costs associated with liposomal technologies and strict regulatory variations across regions, which can hinder global standardization and consumer trust.

At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

doi:10.1038/38738

Others benefit more from the growth factors in PRP