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Lei, J., Kusov, Y

For example, the use of ALDH2 activators may accelerate the detoxification of 4-HNE and reduce cellular damage [130, 147]

In vitro desuccinylation assay Hypersuccinylated GCLC proteins purified from HEK293T cells were incubated with purified SIRT2 in the presence or absence of 1 mmol/L NAD+ at 37 C for 4 h with desuccinylation buffer (50 mmol/L Tris-HCl [pH 7.5], 150 mmol/L NaCl, 1 mmol/L MgCl 2 )

The membrane was blocked with 5% PBST-containing non-fat dry milk (Fisher Scientific) for 30 min to remove any non-specific binding to the antibody

Cell-free mercury(II)-reducing activity in a plasmid-bearing strain of Escherichia coli