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To determine the expression of translocated Nrf2 and NF-B in the nucleus, and the levels of Nrf2, Keap1, heme oxygenase-1 (HO-1) and p-IB in the cytoplasm, nuclear and cytoplasmic extracts from the lung tissue were prepared with a previously described method (Rajendrasozhan et al., 2010)

Gene modulation

Anti-Inflammatory Mechanisms Observed in GHK-Cu Research: Suppression of pro-inflammatory cytokines (e.g., IL-6, TNF-) Activation of antioxidant defense systems Modulation of immune cell activity Support for a healthy skin barrier Reduction of oxidative stress Can GHK-Cu Improve Acne Scars Through Collagen Production and Skin Remodeling

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