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For CYP3A4 inhibition testing, ketoconazole (MilliporeSigma, catalog: PHR1385) was prepared at 2 final concentration in culture medium and added in equal volume to each well of a 384-well plate for 24 h

In light of these findings, it may be necessary to reexamine the current RDAs for adequate Vitamin B6 status in many population groups especially since MTHFR mutations affect B6 metabolism so significantly