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Description

It was found that H241 in the active site plays an important role in PLP binding because it has different conformations in the apo and holo structures of Lb TDC

10.1038/ncomms5172 130 KangS

Proc Natl Acad Sci USA (2005) 102(7):25616

Gonadal and mesenteric adipose tissues were harvested and digested in PBS containing 1 mg/ml of collagenase IV (Worthington, # LS004209) and 20 M HEPES buffer at 37 C on thermoblock by rotating at 750 rpm for 30 minutes