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621 Obesity, metabolic syndrome, diet, lifestyle, genetics, and environmental factors contribute to their development

To isolate fractionated adipocytes, fresh gWAT was minced and digested for 1 h in PBS containing 10 mM CaCl 2 , 2.4 U ml 1 Dispase II (Roche) and 1.5 U ml 1 collagenase D (Roche)