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The physiological effects of these factors are mediated through the activation of two related tyrosine kinase receptors: the insulin receptor (INSR), and the type-I insulin-like growth factor receptor (IGF1R)

This analytical method can be monitored using UV detection at 200 nm

Major challenges using GEMIs are confounding factors like changes in pH which potentially also affect the fluorescence signal as well as the calibration of GEMIs to the actual concentration of the metabolite [101]