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Acta Amaz 13, 737797

Then the pellet was solubilized in buffer containing 20 mM HEPES, pH 7.5, 750 mM NaCl, 1% (w/v) n-dodecyl-b-D-maltoside (DDM, Anatrace), 0.2% (w/v) sodium cholate (Sigma), 0.1% (w/v) cholesterol hemisuccinate (CHS, Anatrace), 20% (v/v) glycerol, 0.2 g/ml leupeptin, 100 g/ml benzamidine, 500 unit Salt Active Nuclease (Arcticzymes) and 1 M PF-05190457 at 4 C for 3 h

In contrast, we found 1,942 upregulated and 310 downregulated genes in scav-4(-) mutants compared to the wild type when both were fed with OP50, suggesting that SCAV-4 plays a physiological role under the regular OP50 diet (Table S3-S4)

Han, D., et al., LKB1/AMPK/mTOR signaling pathway in non-small-cell lung cancer

They can help break down circulating immune complexes, degrade excess fibrin (which is highly relevant for the microclots seen in Long COVID), and modulate systemic cytokine levels