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Colorimetric assay of MDA and GSH in hepatic tissue homogenate Oxidative stress was evaluated by assaying reduced glutathione (GSH) and malondialdehyde (MDA) content in hepatic tissue homogenates using colorimetric kits (Biodiagnostic, Egypt) and following the instructions of the manufacturer

Base excision repair of oxidative DNA damage and association with cancer and aging

Additionally, disruption of the bloodbrain barrier (BBB) occurs through endothelial injury, tight junction breakdown, and matrix metalloproteinase activation, which increases vascular permeability and contributes to cerebral edema and secondary injury (28)

Preparation of nanoparticles P2 (100 mg) or P4 (100 mg) was dissolved in 1 mL DMSO, and the solution were then slowly added dropwise to 10 mL of water