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published that short interfering RNA (siRNA) and Keap1 were involved in silencing the expression of Nrf2 in coronary arterial endothelial cells, leading to cellular proliferative capacity impairment, improper adhesion to extracellular matrix proteins, reduced migration, and impaired capillary formation (Valcarcel-Ares et al., 2012)

The resuspended cells were lysed by sonication using a Branson sonifier (3.2 mm tip) at 45% amplitude, with 10 s on/off pulses, for a total of 3 min

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In disease refractory to these agents, pharmacological augmentation with neuroleptic drugs, which antagonize the D2 dopamine receptor, can be efficacious (M