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Several studies reported lower levels of selenium in smokers [26, 27]

The total RNA from the treated cells was acquired using Trizol reagent (Invitrogen Life Technologies, CA, USA) according to the manufacturers instructions

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Health Serv Res 33: 586588

Therefore, five related pathways of ferroptosis, AMPK signaling, Activating transcription factor 4(ATF4), BECN1 signaling, NOX4 signaling, Yes associated protein/transcription costimulator with PDZ-binding domain (YAP/TAZ), will be discussed below to provide new targets for future disease therapy and new drug research ( Figure 3 )