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the reaction mixture was irradiated at wavelength of 365 nm using a UV lamp at room temperature, with samples removed for LC-MS analysis at 30 minute intervals thereafter

Extraction buffer consisted of 6 mg/mL 5-sulfosalicylic acid dehydrate, 0.1 % v/v Triton X-100 and Complete, EDTA-free Proteinase inhibitor cocktail (Roche) in KPE buffer (0.1 M potassium phosphate buffer and 5 mM EDTA at pH 7.5)

Women demonstrate lower prevalence of T2D and prediabetes in younger ages as well as decreased mortality due to diabetes compared to males despite higher BMI in women