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In another study, GPX4 knockout mice showed significant cognitive impairment and degeneration of hippocampal neurons when challenged with the water maze test

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Flow cytometry analysis For experiments in vitro, cells were trypsinized, fixed with 4% PFA (Fisher Scientific) for 10 min, washed with PBS, and resuspended in FACS buffer (PBS, 1% BSA, 0.5 mM EDTA, and 25 g/ml DNAse)

This is the basis for many online statements claiming that the compound changes body composition without suppressing appetite

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