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Description

85-23, revised 1985) of the National Institutes of Health and the IBC

Microbiota-derived butyrate activates AMPK/Akt signaling and suppresses gluconeogenesis

Sigma-Aldrich, #F6627) twice on day 0 and day 1

After the addition of 2 L of the gel loading buffer (60% glycerol, 10 mM TrisHCl, pH 7.4, 60 mM EDTA, 0.15 % Orange G), the reaction mixtures (3 L) were resolved in 0.8% agarose gel in 1x TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 7.5)