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Product Specifications Specification Detail Product typeCell detachment / dissociation reagent FormatSterile-filtered liquid, ready-to-use Volume100 ml Working concentration1x (ready-to-use) Enzyme activityCombined proteolytic and collagenolytic Enzyme originNon-mammalian and non-bacterial Buffer systemDulbeccos PBS with EDTA pH indicatorPhenol red pH range6.8 7.8 AppearanceClear, pale red to orange solution Storage temperature-15 C or below Stability after thawingUp to 2 months at +2 C to +8 C Recommended use volume~10 ml per 75 cm2 culture surface Typical incubation time5 10 minutes at room temperature Shipping conditionsFrozen on dry ice Intended useFor research use and further manufacturing only Formulation (Composition per Liter) Component Concentration (mg/L) Inorganic Salts Sodium chloride (NaCl)8000.00 Disodium hydrogen phosphate (Na2HPO4)1150.00 Potassium chloride (KCl)200.00 Potassium dihydrogen phosphate (KH2PO4)200.00 Other Components EDTA 4Na (tetrasodium EDTA)220.00 Phenol red3.00 Proprietary enzyme blend (proteolytic and collagenolytic activity)1x Accutase is a registered trademark of Innovative Cell Technologies, Inc

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Fudenberg and coworkers addressed this question from a different viewpoint and asked whether heat-eluted IgG from senescent RBC has the same specificity as anti-Thomson-Friedenreich antibodies (anti-T), that are generated against neuraminidase-treated RBC glycoproteins