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Why AIM Care Matters More Than Ever Recently, we heard the story of Huckleberry, an 18-year-old cat living in New York

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It is widely known that the assembly of the NLRP3 inflammasome leads to the activation of caspase-1, which further results in the production of several pro-inflammatory cytokines

Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells stained double-positively (Annexin V+/PI+) with both FITC and PI