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The suspended brain cubes were centrifuged at 100 g, 4 C for 20 sec to remove medium, and washed once with the medium

Effect of alternate-day fasting on weight loss, weight maintenance, and Cardioprotection among metabolically healthy obese adults: a randomized clinical trial

J Immunol (2012) 188(9):430514

Build gradually if you feel well and recover well

Consequently, many studies report changes of the fluorescence signal of the ATP sensors as proxy for the ATP-concentration, and only few reports provide data on actual, molar concentrations of ATP derived from ATP sensor imaging [64, 102, 103]