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Normality and homogeneity of variances were confirmed by the ShapiroWilk and Levene tests, respectively, before parametric analyses (t-test and ANOVA) were run

If deficiency is confirmed, they will investigate the underlying causewhether dietary insufficiency, malabsorption, or pernicious anaemiato guide appropriate treatment and monitoring strategies

In hypoxic disc environments, hypoxia-inducible factor 1 alpha (HIF-1) activates autophagy pathways, helping to mitigate ROS generation while promoting chondrogenic differentiation and cartilage matrix synthesis [84]