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Rat Glutathione Reductase Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Glutathione Reductase in biofluid samples, such as serum, plasma, tissue homogenates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . GR present in samples reacts with an anti-Rat GR capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-GR detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active GR in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat GR. Detection Target: Rat Glutathione Reductase Reactivity: Rat Range: 0.156-10ng/mL Sensitivity: 0.066ng/mL Storage Conditions: Store all components at 4C This Rat GR activity assay is for the quantitative determination of Glutathione Reductase in samples including serum, plasma, tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results