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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

The relaxation delays used for the T1 experiments were 20, 50, 90, 130, 200, 320, 450, 580, 750, 900, 1200, and 2005 ms

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16 with thiocholine esters as substrates and 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) as the thiol reagent