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Adulteration with synthetic drugs (e.g., corticosteroids in anti-diarrheal preparations) or contamination with heavy metals and pesticides further undermines safety

Gene 67:3140 Editor information Editors and Affiliations Rights and permissions Copyright information 2019 Springer Science+Business Media, LLC, part of Springer Nature About this protocol Cite this protocol Kim, SY., Hakoshima, T

Although the time course of Nrf2 stabilization and nuclear translocation indicated a rapid increase with a peak at approximately 6 h for DEM, and a slow but gradual rise triggered by NFT, maximal accumulation of Nrf2 protein, as well as the induction of GCLC and glutathione synthesis, were almost identical for both NFT and DEM after incubation periods of and beyond 24 h (Fig

Endogenous redox sensors ( e.g

To address this, we additionally deleted the gene coding for Ggt in these cells and analyzed reduction of Grx1-roGFP2 by exogenous GSH in the resulting gshA gsiC ggt triple deletion mutant