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Osteopontin silencing attenuates bleomycin-induced murine pulmonary fibrosis by regulating epithelialmesenchymal transition

Experience of implementing metagenomic next-generation sequencing in patients with suspected pulmonary infection in clinical practice

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Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap) Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2) Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28) Transfect Common Cells Efficiently and Cost-Effectively For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount