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However, there are several major limitations to the current studies that require further investigation: 1

J Clin Med 12(20):6653 Canovas-Ahedo M, Alonso-Alconada D (2019) Combined therapy in neonatal hypoxic-ischaemic encephalopathy

LPS could induce NF- B-mediated inflammation [72] and increase intracellular ROS and activate the ROS/p38/MAPK signaling pathway, which affected apoptosis [73]

After incubation, the membranes were washed three times and then incubated at room temperature for 1 h with secondary antibodies, including IRDye 680RD goat anti-mouse IgG (H + L) (Licor, 926-68070), IRDye 680RD goat anti-rabbit IgG (H + L) (Licor, 926-68071), IRDye 800CW goat anti-mouse IgG (H + L) (Licor, 926-32210), IRDye 800CW goat anti-rabbit IgG (H + L) (Licor, 926-32211)