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Some studies have used endogenous small interfering RNAs (miRNAs) to inhibit NNMT and competitive endogenous RNAs (ceRNAs) as sponges of miRNAs to reduce the inhibitory effect of miRNAs on target genes

Preclinical research evidence Animal Experiments Obese mouse model: After subcutaneous injection of AOD 9604 (250 g/kg) daily for 8 weeks, the body weight of obese mice decreased by 12%, the subcutaneous fat mass decreased by 25%, and the weight of epididymal adipose tissue decreased significantly

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Laboratory research may investigate: enzymatic pathways involved in NAD+ synthesis and recycling interactions between NAD-dependent enzymes and metabolic signaling networks cellular responses to altered nicotinamide availability These investigations are typically conducted in controlled in vitro systems and molecular biology research platforms