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This can denature (destroy) the peptide at the air-liquid interface

ATP restoration experiments revealed that synthesis of new AMP is essential for the rapid restoration of normal ATP levels in ATP-depleted astrocytes and that this AMP synthesis via the purine salvage pathway requires an adenine precursor, a substrate that is efficiently metabolized to provide the energy for efficient ATP regeneration from ADP [60] as well as a suitable substrate that provides the ribose phosphate needed for AMP synthesis [241]

Herting B., Diakonie-Klinikum Schwbisch Hall, Department of Neurology

Stavric B (1988) Methylxanthines: toxicity to humans

Metabolic reprogramming by MYCN confers dependence on the serine-glycine-one-carbon biosynthetic pathway