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The HBSS was removed, and the cells washed four times with ice cold HBSS (pH 7.4), before fresh growth medium without FCS and supplemented with MEM non-essential amino acids (ThermoFisher) was added to one of the plates and incubated ON before samples were collected (recycling fraction)

The second phase indicates loss of the 640 nm charge transfer absorption concomitant with accumulation of the broadly absorbing thiolate-FAD charge transfer

Allowing minoxidil to fully dry before outdoor activities prevents dilution from perspiration

J Plant Growth Regul 37(3):925936

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