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[6] After deep intramuscular injection the lipophilic ester diffuses slowly from the oil depot, undergoes enzymatic cleavage to free testosterone and enters the systemic circulation, where roughly 98 % is reversibly bound to sex-hormone-binding globulin and albumin while the unbound fraction translocates across cell membranes to activate androgen receptors

Three biological replicates for each group were tested and each reaction was performed with three technical replicates

Eosin staining was performed for 3 min, and samples were briefly rinsed under running water before being subjected to an ethanol dehydration gradient

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found that disrupting cellular glycolysis can promote intracellular Cu overload, convert the TME from a suppressive state to an active state, and enhance the ICD induced by cuproptosis, thereby inhibiting tumor growth and distant metastasis, which was achieved using a nano-platform in animal models (Li Q