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For migration assay, transfected A375SM cells were suspended in serum-free MEM and seeded into uncoated transwell upper chamber at 1 10 5 cell/mL of cell density

A high level of TSH suggests that your pituitarys thermostat is reacting to low thyroid hormone levels due to hypothyroidism

Store the reconstituted peptide in the refrigerator

2.2 Production and purification of carnitine monooxygenase The individual subunits CntA and CntB of carnitine monooxygenase were produced and purified as described elsewhere (Massmig et al., 2020)