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100 L of dithiothreitol (DTT) solution (8 mM DTT in urea buffer) was added to the filter unit, covered with tin foil, and incubated for 15 min at 56 C in a dry block heating system to reduce proteins

However, regulations are in flux, and they are likely to change with the emergence of more safety-related data

CHO, Chinese hamster ovary cells

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ACC1 expression has been shown to be frequently upregulated in several tumor types 37 , and have reported the expression and/or activation of ACC2 to be inhibited in a variety of cancers 38