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Experimental conditions evaluate the reactions of the receptor, binding patterns, and concentration-dependent responses in laboratory models

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All drugs were administered by gavage after the TSCI surgery for at most 7 days

J., Li X., Pang H., Pan J

Briefly, cell or tissue protein lysates were assayed in transparent 96-well plates (Greiner 655101) in the presence of 1 mM of the GGT substrate L-glutamic acid -p-nitroanilide (GpNA, Cayman 36209) and 20 mM of the transpeptidation acceptor glycyl-glycine (Sigma-Aldrich G1002) in Tris-HCl 100 mM pH 8