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Based on the multiple sequence alignment and using mutagenesis and activity assays, we have identified for the first time the key catalytic residues of SapM, including the nucleophile S95 and residues (E59, N60, D238 and E239 H160 and H204) which are associated with metal and substrate binding (Fig

The circadian autoregulatory feedback loop At the molecular level, the circadian clock consists of multiple sets of transcription factors resulting in autoregulatory transcription-translation feedback loops (TTFLs) that represent the core mechanism of the circadian clock in mammals

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[DOI] [PMC free article] [PubMed] [Google Scholar] 56.Suzuki T., Yamaguchi H., Kikusato M., Matsuhashi T., Matsuo A., Sato T., Oba Y., Watanabe S., Minaki D., Saigusa D., et al