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Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight

Feldman EL, Goutman SA, Petri S, Mazzini L, Savelieff MG, Shaw PJ, et al

Despite the rapid onset, the maximum analgesic and respiratory depressant effect is achieved only after a few minutes

Additionally, genetic testing results and the cytokine profile in the patients serum are necessary to assess eligibility and tailor the treatment to the individuals specific needs

Integr Med (Encinitas)