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Organ suspensions were subjected to serial 3-, 5- or 6-fold dilutions (depending on the state of disease progression) in modified Schneiders Drosophila medium (Leitherer et al, 2017), with 12 replicates per dilution step

doi: 10.1016/j.ccell.2018.03.004 25 DahmusJDKotlerDLKastenbergDMKistlerCA

For in vivo metabolic analysis, mice were intraperitoneally injected with a 15 N-glutamine solution (40 mg/kg in PBS)

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